Antibodyantigen complexes have been detected by chemiluminescence using ECLTM Choose Western Blotting Detection Package (GE Healthcare) as indicated in manufacturer’s guidelines
In vitro differentiated adipocytes were handled with 40 nM of miRIDIAN miRNA Mimics (Thermo Fisher Scientific, Lafayette, CO) and HiperFect Transfection Reagent (Qiagen, Hilden, Germany) at day ten?2 of differentiation…