The translation inhibitor cycloheximide (closing focus of 20 mg/ml) was utilised to block protein manufacturing to quantify maturation of the vYFP proteins
A yeast strain (K699 MATa ade2-1 trp1-one can 1-100 leu2::LEU2-GAL1pr-VYFP his3-eleven,15 ura3) expressing genomically-inserted Venus yellow fluorescent protein (vYFP) below the management of the inducible GAL1 promoter was generously presented…