To co-express TbKIN-D-PTP and TbKIN-C-3HA in the bloodstream cells for co-immunoprecipitation, wild-variety 221 cells were being first transfected with Computer-TbKIN-D-PTP-NEO and picked with fifteen mg/ml G418
The bloodstream-sort Solitary Marker (SM) cell line was cultured in HMI-9 medium supplemented with 10% fetal bovine serum (Atlanta Biologicals, Inc) and fifteen mg/ml G418 in a 37uC incubator provided…