The tartrate-resistant acid phosphatase (TRACP) optimistic multinucleated cells (3 nuclei) had been counted as osteoclasts by employing Leukocyte Acid Phosphoatase Assay Kit (Sigma-Aldrich)
RAW264.7 cells have been suspended in -MEM with ten% FBS and one hundred ng/ml RANKL and plated on a 96-well Corning Osteo Assay plate at an original density of 103…