In contrast, other proapoptotic Bcl-two proteins induced apoptosis already at 24 h, as demonstrated listed here for the BH3-only protein Bik/Nbk subcloned in the similar adenoviral history (Fig. 1G)
Detailed protocols for protein extraction and Western blot investigation had been explained previously . As a common, 106 cells were being harvested and dissolved in lysis buffer (150 mM NaCl,…