Complete RNA was extracted from mucosa and denuded-detrusor layers of rat bladders using a ReliaPrep RNA Tissue Miniprep program (Promega, Usa) according to manufacturer’s directions
Strips have been equilibrated under a resting tension of one..five g for 60 min. Pressure was monitored by using isometric force transducers (Pioden Controls Ltd, British isles) and a Powerlab…