The length of the colon was measured, the colon was flushed with chilly PBS and opened longitudinally for morphologic research, and tissue obtained from just about every colon was processed for additional assays
In vivo permeability assay to assess barrier function was performed working with an FITC-labeled dextran approach, as described . Briefly, eight- to ten-wk-old mice ended up used. Foodstuff and h2o…