Area labeling of HSlo/mutant in transfected HEK293 cells with Ibtx-LC-biotin was completed as described previously
For S918D and S922D the forward primer was 59 ggacagcatttgccgtcgacgtcctggacgatctcatgagcgcgacg 39 and the backward primer 59 cgtcgcgctcatgagatcgtccaggacgtcgacggcaaatgctgtcc 39. The mutant inserts had been sequenced in their entirety following mutagenesis. b-catenin-EGFP…