Lysates ended up collected 36 h following transfection, and luciferase exercise was measured with the twin luciferase assay (Promega)
The ventricles have been minced with scissors and digested at 37 oC in phosphate-buffered saline (PBS) containing .one% trypsin (Gibco BRL) and .05% kind I collagenase (Gibco BRL). After eight-10…