As shown in Determine 6C, only monomeric but not multimeric p35bac induced cellular proliferation of Ba/F3-gp130-IL-12Rb1-IL-12Rb2 cells when combined with supernatant containing p40C197A
Ba/F3-gp130-IL-12Rb1-IL-twelve-Rb2 cells proliferated with recombinant p35bac and p35bac/C92A supplemented with consistent amounts of p40C197A conditioned supernatant from HEK293 cells. As unfavorable controls, p35bac and p35bac/C92A or p40 alone did not…