UC, 10 min. at 95uC, followed by 45 cycles of 15 s at 95uC
UC, 10 min. at 95uC, followed by 45 cycles of 15 s at 95uC and 1 min. at 60uC. Results were analyzed with the SDS 7900 system software, version 2.1…
UC, 10 min. at 95uC, followed by 45 cycles of 15 s at 95uC and 1 min. at 60uC. Results were analyzed with the SDS 7900 system software, version 2.1…
D alveolar macrophages using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA) and NF-kB DNA 56-59-7 site binding activity was measured by EMSA as described . Briefly, nuclear proteins were…
Ulation of cellular Zn2+ levels. The sensors were then targeted to both the nucleus and cytosol and nuclear sensors were used in conjunction with an organelle-localized CFPYFP-based Zn2+ sensor to…
Y (Fig. 4). Codon optimization has been established as an efficient measure to overcome the bias on codon usage frequency and significantly improve the expression of foreign gene in Pichia…
Rane protein Tom6 .Fusion AssayCells of opposing mating type (expressing green or red fluorescent proteins) were grown separately (12?6 h, log phase) in YPGALA, transferred to YPGA, mixed and incubated…
Elative to the total CDC25A transcripts in NSCLC cell lines and tissue samples, the “Total” real time-PCR assay determines total CDC25A template in reaction (Cttot) while the “wt” real time-PCR…
Proteins are required for cell survival. Normally, GRP78 binds PERK, IRE1 and ATF6 and inhibits their activation in non-stressed cells . When unfolded proteins in the ER lumen reach a…