1 6 1 2 1 5 8 3hpx hpx wap65-2 ybxJZ575434 JZ575433 JZ575518 JZ3E-28 1E-31 9E-15 6E-5 19 36Unclassified Unclassified Unclassified UnclassifiedPLOS ONE | DOI:10.1371/journal.pone.0121224 March 30,16 /Differential Gene Expression in the Liver of the African LungfishTable 5. Known transcripts found in the reverse SSH library (down-regulation) obtained by suppression subtractive hybridization PCR from the liver of Protopterus annectens after 1 s11606-015-3271-0 day of arousal from 6 months of aestivation with fish aestivated for 6 months in air as the reference for comparison. Group and Gene Carbohydrate metabolism fructose-bisphosphate aldolase B fragment 2 plasma alpha-L-fucosidase precursor putative Protein synthesis, transport and folding 60S ribosomal protein L32 60S ribosomal protein L35 60S ribosomal protein L36 eukaryotic translation elongation factor 2 Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) 1471-2474-14-48 ubiquitously expressed (fox derived) ribosomal protein L12 ribosomal protein L17 ribosomal protein L19 ribosomal protein L23 ribosomal protein L27a ribosomal protein L3 ribosomal protein L30 ribosomal protein L32 ribosomal protein L34 ribosomal protein L36 ribosomal protein L38 ribosomal protein L6 ribosomal protein L7a-like fragment 2 ribosomal protein L9 ribosomal protein Large P0 ribosomal protein S11 ribosomal protein S12 fragment 2 ribosomal protein S15 ribosomal protein S2 fragment 2 ribosomal protein S24 ribosomal protein S27a ribosomal protein S9 rpl32 rpl35 rpl36 eef2 fau JZ575383 JZ575384 JZ575385 JZ575415 JZ575421 Xenopus (A-836339 structure Silurana) tropicalis Xenopus (Silurana) tropicalis Xenopus laevis Xenopus (Silurana) tropicalis Xenopus (Silurana) tropicalis Salmo salar Protopterus dolloi Protopterus dolloi Xenopus (Silurana) tropicalis Xenopus laevis Xenopus (Silurana) tropicalis Xenopus laevis Xenopus (Silurana) tropicalis Xenopus laevis Xenopus laevis Danio rerio Salmo salar Protopterus dolloi Xenopus laevis Protopterus dolloi Xenopus (Silurana) tropicalis Xenopus laevis Xenopus (Silurana) tropicalis Danio rerio Xenopus (Silurana) tropicalis Xenopus (Silurana) tropicalis Protopterus dolloi 2E-92 4E-83 8E-60 9E-27 3E-39 1 7 2 6 3 Translation Translation Translation Translation Translation aldob fuca JZ575423 JZ575460 Protopterus annectens Salmo salar 9E145 7E-10 7 2 Glycolysis Carbohydrate metabolic process, fucose metabolic process Gene symbol P. annectens accession no. Homolog species Evalue No of clones Biological processesrpl12 rpl17 rpl19 rpl23 rpl27a rpl3 rpl30 rpl32 rpl34 rpl36 rpl38 rpl6 rpl7a rpl9 rplp0 rps11 rps12 rps15 rps2 rps24 rps27a rpsJZ575472 JZ575473 JZ575474 ONO-4059 web JZ575475 JZ575478 JZ575467 JZ575479 JZ575480 JZ575481 JZ575482 JZ575483 JZ575468 JZ575470 JZ575471 JZ575485 JZ575491 JZ575493 JZ575494 JZ575488 JZ575495 JZ575496 JZ6E-04 3E136 2E-99 1E110 2E-80 2E118 1E123 4E100 4E-82 2E-77 1E-50 3E-10 2E-74 2E-67 0 2E113 2E-41 6E112 5E-70 4E-90 1E1124 3 3 2 2 9 5 1 2 10 1 1 8 4 4 5 1 4 8 2 1Translation Translation Translation Translation Translation Translation Translation Translation Translation Translation Translation Translation Ribosome biogenesis Translation Translational elongation Translation Translation Translation Translation Translation Translation Translation (Continued)PLOS ONE | DOI:10.1371/journal.pone.0121224 March 30,17 /Differential Gene Expression in the Liver of the African LungfishTable 5. (Continued) Group and Gene Signaling alpha fetoprotein rho GTPase activating protein 29 secretogranin II Structure thymosin-beta 4 tms.1 6 1 2 1 5 8 3hpx hpx wap65-2 ybxJZ575434 JZ575433 JZ575518 JZ3E-28 1E-31 9E-15 6E-5 19 36Unclassified Unclassified Unclassified UnclassifiedPLOS ONE | DOI:10.1371/journal.pone.0121224 March 30,16 /Differential Gene Expression in the Liver of the African LungfishTable 5. Known transcripts found in the reverse SSH library (down-regulation) obtained by suppression subtractive hybridization PCR from the liver of Protopterus annectens after 1 s11606-015-3271-0 day of arousal from 6 months of aestivation with fish aestivated for 6 months in air as the reference for comparison. Group and Gene Carbohydrate metabolism fructose-bisphosphate aldolase B fragment 2 plasma alpha-L-fucosidase precursor putative Protein synthesis, transport and folding 60S ribosomal protein L32 60S ribosomal protein L35 60S ribosomal protein L36 eukaryotic translation elongation factor 2 Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) 1471-2474-14-48 ubiquitously expressed (fox derived) ribosomal protein L12 ribosomal protein L17 ribosomal protein L19 ribosomal protein L23 ribosomal protein L27a ribosomal protein L3 ribosomal protein L30 ribosomal protein L32 ribosomal protein L34 ribosomal protein L36 ribosomal protein L38 ribosomal protein L6 ribosomal protein L7a-like fragment 2 ribosomal protein L9 ribosomal protein Large P0 ribosomal protein S11 ribosomal protein S12 fragment 2 ribosomal protein S15 ribosomal protein S2 fragment 2 ribosomal protein S24 ribosomal protein S27a ribosomal protein S9 rpl32 rpl35 rpl36 eef2 fau JZ575383 JZ575384 JZ575385 JZ575415 JZ575421 Xenopus (Silurana) tropicalis Xenopus (Silurana) tropicalis Xenopus laevis Xenopus (Silurana) tropicalis Xenopus (Silurana) tropicalis Salmo salar Protopterus dolloi Protopterus dolloi Xenopus (Silurana) tropicalis Xenopus laevis Xenopus (Silurana) tropicalis Xenopus laevis Xenopus (Silurana) tropicalis Xenopus laevis Xenopus laevis Danio rerio Salmo salar Protopterus dolloi Xenopus laevis Protopterus dolloi Xenopus (Silurana) tropicalis Xenopus laevis Xenopus (Silurana) tropicalis Danio rerio Xenopus (Silurana) tropicalis Xenopus (Silurana) tropicalis Protopterus dolloi 2E-92 4E-83 8E-60 9E-27 3E-39 1 7 2 6 3 Translation Translation Translation Translation Translation aldob fuca JZ575423 JZ575460 Protopterus annectens Salmo salar 9E145 7E-10 7 2 Glycolysis Carbohydrate metabolic process, fucose metabolic process Gene symbol P. annectens accession no. Homolog species Evalue No of clones Biological processesrpl12 rpl17 rpl19 rpl23 rpl27a rpl3 rpl30 rpl32 rpl34 rpl36 rpl38 rpl6 rpl7a rpl9 rplp0 rps11 rps12 rps15 rps2 rps24 rps27a rpsJZ575472 JZ575473 JZ575474 JZ575475 JZ575478 JZ575467 JZ575479 JZ575480 JZ575481 JZ575482 JZ575483 JZ575468 JZ575470 JZ575471 JZ575485 JZ575491 JZ575493 JZ575494 JZ575488 JZ575495 JZ575496 JZ6E-04 3E136 2E-99 1E110 2E-80 2E118 1E123 4E100 4E-82 2E-77 1E-50 3E-10 2E-74 2E-67 0 2E113 2E-41 6E112 5E-70 4E-90 1E1124 3 3 2 2 9 5 1 2 10 1 1 8 4 4 5 1 4 8 2 1Translation Translation Translation Translation Translation Translation Translation Translation Translation Translation Translation Translation Ribosome biogenesis Translation Translational elongation Translation Translation Translation Translation Translation Translation Translation (Continued)PLOS ONE | DOI:10.1371/journal.pone.0121224 March 30,17 /Differential Gene Expression in the Liver of the African LungfishTable 5. (Continued) Group and Gene Signaling alpha fetoprotein rho GTPase activating protein 29 secretogranin II Structure thymosin-beta 4 tms.