Uret et al. This pceABCT operon is conserved in the identical genome context in all 5 Dehalobacter genomes,though considerable sequence variations within this operon exists between these five Dehalobacter strains,indicating that the pceABCT operon has been carried by every Dehalobacter for some time (Figure S). Interestingly,within the genomes of strain CF and strain DCA,this operon is positioned in a area flanked by two direct repeats ( bp every) with other repeat patterns,indicating that the operon could happen to be acquired horizontally. These repeat patterns were not discovered in strain PERK nor in strain E. No other proof for the presence of genomic islands or horizontal gene transfer events involving rdhA genes was located in any with the five Dehalobacter genomes. Horizontal gene transfer appears to play an important role in shaping Dehalobacter genomes. As indicated previously,significant sequence variations between the 5 genomes are located in BTZ043 chemical information regions A,B,and C highlighted in Figure . Area A features a current insertion of a kb fragment (A) in the genome of strain CF (Figure. This region has a relatively low GC contentFrontiers in Microbiology www.frontiersin.orgFebruary Volume ArticleTang et alparative Dehalobacter Genome AnalysisFIGURE 3 potential genome rearrangements amongst Dehalobacter restrictus strain PERK and Dehalobacter sp. strain CF. Note the blocks with identical color represent corresponding locations between the genomes of strain CF and strain PERK with higher similarity. Panel (A) shows the Mauve alignment and original GC skew profile from the two genomes. Panels (B represent the step artificial reversion scenario that could explain the genome rearrangements observed amongst the two strains. Actions and in Panels (B,C) describe two sequence inversions,and step in Panel (D) represents a translocation. The resulting modifications in Mauve alignments are depicted with corresponding changes in GC skew profiles shown for the correct.( and most genes involved are exceptional to strain CF. The insertion is probably related to a phage integrase (DCF_p) positioned at the finish of A (Figure. In Region C,which features a low GC content (strain CF has numerous phagerelated genes. Incorporation of region C (Figure into strain CF is most likely associated to a sitespecific recombinase (DCF_p)positioned at the finish of C. Alternatively,sequence C from strain DCA is kb extended and has a GC content of . ,comparable for the typical GC content material from the whole genome,and may possibly indicate that C is native to Dehalobacter. In area B,both B (strain CF) and B (Strain DCA) possess a low GC content,and poor sequence conservation was located amongst allFrontiers in Microbiology www.frontiersin.orgFebruary Volume ArticleTang et alparative Dehalobacter Genome Analysisfive Dehalobacter genomes,indicating a hypervariable region. Area D (Figure is one more massive phage elated area shared by both strain CF and DCA,but not found in strain PERK and E. Region D is kb extended and contains many genes encoding either phagerelated or hypothetical proteins,such as a phage integrate situated in the end of your region. This insertion event targeted tRNAThr (DCF_r) and resulted in duplication ( bp) in the insertion web site,which consists of a partial tRNAThr gene.Metabolic PotentialWe examined in detail the gene annotations of Dehalobacter to reveal metabolic prospective and any differences among the strains. All round,these Dehalobacter strains are hugely comparable. Genes for PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20972551 all key metabolic pathways and physiological specifications of.