And a.C. All authors have study and agreed towards the published version of the manuscript. Funding: This analysis was in element funded by Sapienza Universitdi Roma, grant number RP120172B49BE241 to S.P. Institutional Overview Board Statement: Not applicable. Informed Consent Statement: Not applicable. Data Availability Statement: Publicly readily available datasets have been analyzed in this study. Information presented in this study are out there on request from the corresponding authors. Conflicts of Interest: The authors declare no conflict of interest.
biomoleculesArticleMolecular Characterization and Defense Functions from the Nile Tilapia (Oreochromis niloticus) DnaJ B9b and DnaJ C3a Genes in Response to Pathogenic Bacteria beneath High-Temperature Stress ConditionsPrapansak Srisapoome 1,two,3, , Kubpaphas Thummabancha 1,2,three and Ratree WongpanyaLaboratory of Aquatic Animal Overall health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, Thailand; [email protected] Center of Sophisticated Studies for Agriculture and Meals, Kasetsart University Institute for Sophisticated Research, Kasetsart University, Bangkok 10900, Thailand Center of Excellence in Aquatic Animal Well being Management, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, Thailand Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; [email protected] Correspondence: [email protected]; Tel.: +66-2579-Citation: Srisapoome, P.; Thummabancha, K.; Wongpanya, R. Molecular Characterization and Defense Functions of the Nile Tilapia (Oreochromis niloticus) DnaJ B9b and DnaJ C3a Genes in Response to Pathogenic Bacteria below High-Temperature Pressure Situations. Biomolecules 2021, 11, 1509. https:// doi.org/10.3390/biom11101509 Academic Editor: Hidetaka Torigoe Received: 25 August 2021 Accepted: ten October 2021 Published: 13 OctoberAbstract: DnaJ proteins or heat shock protein 40s (HSP40s) form certainly one of the largest heat shock protein families. In this study, two cDNAs encoding Nile tilapia (Oreochromis niloticus) DnaJ proteins (On-DnaJ B9b and On-DnaJ C3a) have been successfully cloned and characterized. The structures and organizations of those two genes are initially reported inside the present study. On-DnaJ B9b is roughly 2.1 kb lengthy and contains two exons and 1 intron, while On-DnaJ C3a is about 12 kb lengthy and contains 12 exons and 11 introns. Under typical conditions, On-DnaJ B9b mRNA is extremely Trilinolein Protocol expressed in gonad and trunk kidney tissues, even though On-DnaJ C3a transcripts are abundantly expressed in gills, intestine, liver, and trunk kidney tissues. Following pathogenic infections, the expression of both genes is induced in the liver, spleen and head kidney tissues of Nile tilapia that had been infected with two virulent pathogenic bacteria, Streptococcus agalactiae and Flavobacterium columnare. Silencing of those two genes was initially carried out, as well as the final results clearly indicated their important roles below each heat and bacterial tension situations. The fundamental expertise obtained from this study indicates the characteristic basic biofunctions of heat shock proteins within the regulation of intracellular proteins throughout infection, which involve stopping protein GW-870086 MedChemExpress aggregation, promoting protein refolding, and activating unfolded protein degradation. Search phrases: molecular chaperone; heat shock protein; Hsp40; aquaculture; Streptococcosis; columnaris; RNAiPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in.