Ymer, Video S2: Flammability test for CNF composite (30 vol ), Video S
Ymer, Video S2: Flammability test for CNF composite (30 vol ), Video S3: Flammability test for CNF composite (55 vol ), Video S4: More flammability test for CNF composite (80 vol ). Author Contributions: Conceptualization, T.S., L.A.B. and W.S.; experimental style, investigation, W.S. W.S. mostly wrote the manuscript with contributions from S.F., L.A.B. and T.S. All authors have read and agreed to the published version of the manuscript. Funding: This BMS-8 Inhibitor Analysis was funded by JST-Mirai R D Plan (JPMJMI17ED), JSPS Grant-inAids for Scientific Analysis (A) (21H04733), and JSPS Grant-in-Aids for Young Scientists (19J22463, 20K15567). LB is supported by the European Analysis Council (ERC), grant agreement No. 742733. Information Availability Statement: The data that assistance the findings of this study are offered in the corresponding authors upon reasonable request. Acknowledgments: TEMPO-oxidized pulp was kindly supplied by DKS Co. Ltd. Conflicts of Interest: The authors declare no conflict of interest.
nanomaterialsArticleQuantitative Estimation of Cell-Associated Silver Nanoparticles Working with the Normalized Side Scattering Intensities of Flow CytometryYoo Jin Shim 1, , My Kieu Ha 1,, and Tae Hyun Yoon 1,two,three, 2Department of Chemistry, College of All-natural Sciences, Hanyang University, Seoul 04763, Korea; [email protected] (Y.J.S.); [email protected] (M.K.H.) Institute of Subsequent Generation Material Style, Hanyang University, Seoul 04763, Korea Research Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Korea Correspondence: [email protected]; Tel.: 82-2-2220-4593 These authors contributed equally to this perform. Present Address: Vaccine Infectious Illness Institute, University of Antwerp, 2610 Antwerpen, Belgium.Citation: Shim, Y.J.; Ha, M.K.; Yoon, T.H. Quantitative Estimation of Cell-Associated Silver Nanoparticles working with the Normalized Side Scattering Intensities of Flow Cytometry. Nanomaterials 2021, 11, 3079. https:// doi.org/10.3390/nanoAbstract: Quantification of cellular nanoparticles (NPs) is among the most Bafilomycin C1 medchemexpress significant methods in studying NP ell interactions. Right here, a basic approach for the estimation of cell-associated silver (Ag) NPs in lung cancer cells (A549) is proposed according to their side scattering (SSC) intensities measured by flow cytometry (FCM). To estimate cellular Ag NPs linked with A549 cells over a broad range of experimental situations, we measured the normalized SSC intensities (nSSC) of A549 cells treated with Ag NPs with five unique core sizes (i.e., 4000 nm, positively charged) under a variety of exposure situations that reflect diverse scenarios of agglomeration, diffusion, and sedimentation in cell culture media, including upright and inverted configurations with unique media heights. Then, we correlated these nSSC values together with the numbers of cellular Ag NPs determined by inductively coupled plasma mass spectrometry (ICPMS) as a well-established cross-validation technique. The distinctive core sizes of Ag NPs and the numerous exposure conditions tested in this study confirmed that the FCM-SSC intensities are very correlated with their core sizes too as the amount of cellular Ag NPs over a linear variety up to 80,000 Ag NPs/cell and 23 nSSC, which can be considerably broader than these of previous studies. Keywords and phrases: cellular association of nanoparticles; flow cytometry; silver nanoparticlesAcademic Editor: Vincenzo Amendola Received: 15 October 2021 Accepted: 10 November 2021 Published:.