Herapeutic targets for inflammation-associated behavioural disorders.OWP3.05 = PF01.Comparison of generic fluorescent dyes for detection of extracellular vesicles by flow cytometry Leonie de Rond1; Edwin van der Pol2; Chi M. Hau3; Zoltan Varga4; Auguste Sturk5; Ton G. van Leeuwen2; Rienk Nieuwland5; Frank A.W CoumansOWP3.04 = PS09.Extracellular vesicles deformation on surface: some tracks to limit it Ksenia Maximova1; Sameh Obeid2; Thierry Burnouf3; Wilfrid Boireau1; Celine Elie-caille1 FEMTO-ST Institute, UBFC, Besancon, France; 2French National Institute for Agricultural Investigation INRA, Rennes, France; 3College of Biomedical Engineering Taipei Medical University, Taipei, Taiwan, Tapei, Taiwan (Republic of China)Academic Health-related Center, University of Amsterdam, Amsterdam, The Netherlands; 2Biomedical Engineering Physics, Academic Health-related Center, University of Amsterdam, Amsterdam, The Netherlands, Amsterdam, The Netherlands; Serine/Threonine Kinase 3 Proteins Biological Activity 3Laboratory Experimental Clinical Chemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, Amsterdam, The Netherlands; 4Biological Nanochemistry Study Group, Institute of Supplies and Environmental Chemistry, Research Centre for All-natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary, Budapest, Hungary; 5Laboratory of Experimental Clinical Chemistry, and Vesicle Observation Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, Amsterdam, The Netherlands; six Division of Biomedical Engineering and Physics, and Vesicle Observation Center, Academic Healthcare Centre of your University of Amsterdam, Amsterdam, The NetherlandsBackground: Despite the booming development of several characterization techniques of extracellular vesicles (EVs), trustworthy nanocharacterization from the EVs nonetheless remains a challenge because of the huge range of their size and cell origin. Methods: In this context, our efforts are aimed at the development of a NanoBioAnalytical (NBA) platform, which combines a number of characterization techniques, including atomic force microscopy (AFM) – a supply of data about EVs metrology. Our principle objective is always to make a versatile biochip nstrument interface, which opens the possibility to multi-technique and multi-scale investigations that in its turn bring comprehensive facts concerning the various EVs populations. Our NBA platform consists in a biochip, which is biofunctionalized in a multiplexed format, by way of the grafting of different relevant and particular ligands. This biochip behaves like a “EVs wise carrier”, since it first enables the biodetection and capture of EVs subsets, due to a surface plasmon resonance instrument, whilst EVs size and morphology are achieved on the identical biochip by AFM in the subsequent spot. Outcomes: Nevertheless, EVs are recognized to be soft and deformable, thus their dimensions and morphology obtained by AFM measurements could differ, among other issues, according to support constraints. Based on no matter if EVs very simple “passive” adsorption or immunocapture on a substrate, as well as function of your antibody density SRSF Protein Kinase 3 Proteins web grafted on it, EVs could deform just about and possibly loose partly their functionality. Additionally, a number of AFM imaging modes and parameters can also impact the metrological analysis of EVs, a few of them becoming definitely critical to warrant a confident EVs nanocharacterization. Ultimately, taking care about these surface and imaging experimental situations, a correlation amongst 2D (on the surface) an.