Name : Human FGFR3 alpha (IIIb) Protein
Product Source :
Recombinant Human FGFR3 (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu23-Gly377.[Accession | P22607-2]
Molecular Weight :
The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FGFR3 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human FGFR3 alpha (IIIb) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FGFR3 alpha (IIIb) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Background :
Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain.FGFR3 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts.
Synonyms :
ACH; CD333; CEK; CEK2; EC 2.7.10; FGF R3; FGFR3; HSFGFR3EX; JTK4
References & Citations :
(1)Hadari Y , Schlessinger J. FGFR3-targeted mAb therapy for bladder cancer and multiple myeloma[J]. The Journal of clinical investigation, 2009, 119(5):1077-1079.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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