Name : Human FGFR2 beta (IIIb) Protein
Product Source :
Recombinant Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg152-Glu378.[Accession | P21802-3]
Molecular Weight :
The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FGFR2 beta (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FGFR2 beta (IIIb) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). Blocking Data Serial dilutions of Anti-FGFR2 Antibody were added into Human FGFR2 beta (lllb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 1.74ng/ml. SPR Data Anti-FGFR2 Antibody, hFc Tag can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 1.98nM as determined in a SPR assay (Biacore T200).
Background :
Four distinct genes encoding closely related FGF receptors, FGF R1 – 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 – 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.
Synonyms :
Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM
References & Citations :
(1)Katoh, Masaru. FGFR inhibitors: Effects on cancer cells, tumor microenvironment and whole-body homeostasis (Review)[J]. International Journal of Molecular Medicine, 2016.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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