Name : Cynomolgus FOLR2 Protein
Product Source :
Recombinant Cynomolgus FOLR2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln9-His187.[Accession | A0A2K5U027-1]
Molecular Weight :
The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM Glycine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus FOLR2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus FOLR2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus FOLR2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
Background :
FOLR1 and FOLR2 are equipped with cellular glycosylphosphatidylinositol (GPI) anchors. FOLR1 is secreted from epithelia with or without a micelle-encapsulated GPI-anchor into milk and other body fluids/secretions, e.g. semen where its interaction with spermatozoa indicates a role in male fertility. FOLR1 and FOLR2 serve as serum biomarkers of various diseases. FOLR3 possesses no GPI-anchor and originates from secretory granules of neutrophil granulocytes; its concentration in serum correlates to the FOLR3 content in leukocytes and rises with increased leukocyte counts (infection, malignancy and pregnancy).
Synonyms :
FBP;FOLR2;OLR2;BETA-HFR;BETA HFR; FR-BETA;FR BETA; FR-P3; FR P3; β-HFR; β HFR; FR-β; FR β
References & Citations :
(1) Holm J, Hansen SI. Characterization of soluble folate receptors (folate binding proteins) in humans. Biological roles and clinical potentials in infection and malignancy. Biochim Biophys Acta Proteins Proteom. 2020 Oct;1868(10):140466. doi: 10.1016/j.bbapap.2020.140466. Epub 2020 Jun 9. PMID: 32526472.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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