Name : Mouse FOLR4/Juno Protein
Product Source :
Recombinant Mouse FOLR4/Juno Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly20-Gly222.[Accession | Q9EQF4-1]
Molecular Weight :
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse FOLR4/Juno on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse FOLR4/Juno is greater than 95% as determined by SEC-HPLC.
Background :
Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno’s presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor.
Synonyms :
Folate receptor 4; FR-delta; FR delta; Izumo1r; Folbp3; Juno; FOLR4, LOC390243
References & Citations :
(1) Grayson P. Izumo1 and Juno: the evolutionary origins and coevolution of essential sperm-egg binding partners. R Soc Open Sci. 2015 Dec 16;2(12):150296. doi: 10.1098/rsos.150296. PMID: 27019721; PMCID: PMC4807442.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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