Name : Human FSTL3 Protein
Product Source :
Recombinant Human FSTL3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Met27-Val263.[Accession | O95633-1]
Molecular Weight :
The protein has a predicted MW of 26.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FSTL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human FSTL3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human Activin A, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human FSTL3, His Tag with the EC50 of 7.6ng/ml determined by ELISA. Cell Based Assay Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.
Background :
Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.
Synonyms :
FLRG; FLRGFSRP; Follistatin-like 3; FSTL3
References & Citations :
(1) Kralisch S, Hoffmann A, Klöting N, Bachmann A, Kratzsch J, Stolzenburg JU, Dietel A, Beige J, Anders M, Bast I, Blüher M, Zhang MZ, Harris RC, Stumvoll M, Fasshauer M, Ebert T. FSTL3 is increased in renal dysfunction. Nephrol Dial Transplant. 2017 Oct 1;32(10):1637-1644. doi: 10.1093/ndt/gfw472. PMID: 28339962.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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