Name : Human GDNF Protein
Product Source :
Recombinant Human GDNF Protein is expressed from E.coli without tag It contains Ser78-Ile211.[Accession | P39905-1]
Molecular Weight :
The protein has a predicted MW of 15.06 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human GDNF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human GDNF is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 5.0ng/ml determined by ELISA.
Background :
Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.
Synonyms :
Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor (ATF)
References & Citations :
Cintrón-Colón AF, Almeida-Alves G, Boynton AM, Spitsbergen JM. GDNF synthesis, signaling, and retrograde transport in motor neurons. Cell Tissue Res. 2020 Oct;382(1):47-56. doi: 10.1007/s00441-020-03287-6. Epub 2020 Sep 8. PMID: 32897420; PMCID: PMC7529617.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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