Name : Mouse GFRA3 Protein
Product Source :
Recombinant Mouse GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asn29-Arg379.[Accession | O35118]
Molecular Weight :
The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 47-52 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse GFRA3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse GFRA3 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse ARTN, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 32.2ng/ml determined by ELISA.
Background :
GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.
Synonyms :
GDNFR-alpha-3; GFR-alpha-3; GFRA3; GFR alpha3; GPI-linked receptor
References & Citations :
(1) Souza RP, Romano-Silva MA, Lieberman JA, Meltzer HY, MacNeil LT, Culotti JG, Kennedy JL, Wong AH. Genetic association of the GDNF alpha-receptor genes with schizophrenia and clozapine response. J Psychiatr Res. 2010 Aug;44(11):700-6. doi: 10.1016/j.jpsychires.2010.01.002. Epub 2010 Jan 29. PMID: 20116071.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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