Name : Rat GFRAL/GFR alpha-like Protein
Product Source :
Recombinant Rat GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag at the N-terminus. It contains Gln20-Gly350.[Accession | D3ZB94]
Molecular Weight :
The protein has a predicted MW of 38.17 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Rat GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Rat GFRAL is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Rat GFRAL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Background :
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Synonyms :
GFR alpha-like; GFRAL; GRAL; C6orf144
References & Citations :
(1)Yang L, Chang CC, Sun Z, et al. GFRAL is the receptor for GDF15 and is required for the anti-obesity effects of the ligand. Nat Med. 2017;23(10):1158-1166. doi:10.1038/nm.4394
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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