Name : Human CD42b/GP1BA Protein
Product Source :
Recombinant Human CD42b/GP1BA Protein is expressed from HEK293 with His tag at the C-Terminus. It contains His17-Leu531.[Accession | P07359]
Molecular Weight :
The protein has a predicted MW of 57.6 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD42b on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD42b is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD42b, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD42b Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Background :
Absence of the CD42 complex leads to the Bernard-Soulier syndrome (BSS), which is a bleeding disorder characterized by thrombocytopenia and giant platelets. So far, mutations have been found in the genes encoding CD42a, CD42b and CD42c in patients with BSS**.
Synonyms :
GP-Ib alpha; GPIb-alpha; GPIbA; GP1BA; BDPLT1; BDPLT3; BSS; CD42B; CD42b-alpha; DBPLT3; GP1B; VWDP; BP1BA; MGC34595
References & Citations :
(1) Clemetson KJ,McGregor JL,James E,Dechavanne M,Luscher EF Characterization of the platelet membrane glycoprotein abnormalities in Bernard-Soulier syndrome and comparison with normal by surface-labeling techniques and high-resolution two-dimensional gel electrophoresis. J Clin Invest 1982 70:304
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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