Name : Human APOA1 Protein
Product Source :
Recombinant Human APOA1 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Asp25-Gln267.[Accession | P02647]
Molecular Weight :
The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human APOA1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human APOA1 is greater than 95% as determined by SEC-HPLC.
Background :
Apolipoprotein A1 (ApoA1) is a main protein moiety in high-density lipoprotein (HDL) particles. Generally, ApoA1 and HDL are considered as atheroprotective. In prooxidant and inflammatory microenvironment in the vicinity to the atherosclerotic lesion, ApoA1/HDL are subjected to modification. The chemical modifications such as oxidation, nitration, etc result in altering native architecture of ApoA1 toward dysfunctionality and abnormality.
Synonyms :
Apo-AI; ProapoA-I; Apoa1; Apolipoprotein A-I; APOA1; MGC117399; Alp-1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2
References & Citations :
(1) Chistiakov DA, Orekhov AN, Bobryshev YV. ApoA1 and ApoA1-specific self-antibodies in cardiovascular disease. Lab Invest. 2016 Jul;96(7):708-18. doi: 10.1038/labinvest.2016.56. Epub 2016 May 16. PMID: 27183204.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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