Name : Biotinylated Human GPRC5D Protein-Nanodisc
Product Source :
Recombinant Biotinylated Human GPRC5D Protein-Nanodisc is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Met1-Val345.[Accession | Q9NZD1-1]
Molecular Weight :
The protein has a predicted MW of 42.10 kDa.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
ELISA Data Immobilized Anti-GPRC5D Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPRC5D Nanodisc, His Tag with the EC50 of 0.12μg/ml determined by ELISA (QC Test). ELISA Data Immobilized Human CD3E&CD3D, His Tag at 2μg/ml (100μl/well) on the plate, add serial dilutions of Anti-Human CD3×GPRC5D Bispecific Antibody, hFc Tag and then add Biotinylated Human GPRC5D Nanodisc, His Tag at 5μg/ml. Detection was performed using HRP-conjugated streptavidin with the EC50 of 0.28μg/ml determined by ELISA. SPR Data Biotinylated Human GPRC5D Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GPRC5D Antibody with an affinity constant of 6.30 nM as determined in SPR assay (Biacore T200). BLI Data Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on SA-Biosensor, can bind Anti-GPRC5D Antibody with an affinity constant of 1.16 nM as determined in BLI assay (Gator Prime).
Background :
Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.
Synonyms :
GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor
References & Citations :
(1) Smith EL, Harrington K, Staehr M, Masakayan R, Jones J, Long TJ, Ng KY, Ghoddusi M, Purdon TJ, Wang X, Do T, Pham MT, Brown JM, De Larrea CF, Olson E, Peguero E, Wang P, Liu H, Xu Y, Garrett-Thomson SC, Almo SC, Wendel HG, Riviere I, Liu C, Sather B, Brentjens RJ. GPRC5D is a target for the immunotherapy of multiple myeloma with rationally designed CAR T cells. Sci Transl Med. 2019 Mar 27;11(485):eaau7746. doi: 10.1126/scitranslmed.aau7746. PMID: 30918115; PMCID: PMC7508042.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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