Name : Human G-CSF R/CD114 Protein
Product Source :
Recombinant Human G-CSF R/CD114 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu25-His627.[Accession | Q99062-1]
Molecular Weight :
The protein has a predicted MW of 68.29 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human G-CSF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human G-CSF R is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human G-CSF R, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Background :
The Granulocyte Colony-Stimulating Factor (G-CSF) receptor, a member of the hematopoietin cytokine receptor superfamily, functions as a homodimer and requires the recruitment of cytosolic protein tyrosine kinases (PTKs) to transduce its signal.
Synonyms :
G-CSF-R; G-CSF R; CD114; G CSF R; CSF3R; Csfgr; GCSFR
References & Citations :
Sampson M, Zhu QS, Corey SJ. Src kinases in G-CSF receptor signaling. Front Biosci. 2007 Jan 1;12:1463-74. doi: 10.2741/2160. PMID: 17127394.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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