Name : Human GITR/TNFRSF18 Protein
Product Source :
Recombinant Human GITR/TNFRSF18 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln26-Glu161.[Accession | Q9Y5U5-1]
Molecular Weight :
The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human GITR is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human GITR Ligand Trimer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 0.44μg/ml determined by ELISA.
Background :
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
References & Citations :
(1)Knee D A , Hewes B , Brogdon J L. Rationale for anti-GITR cancer immunotherapy[J]. European Journal of Cancer, 2016, 67:1-10.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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