Name : Mouse HPX Protein
Product Source :
Recombinant Mouse HPX Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser24-Gln460.[Accession | Q91X72]
Molecular Weight :
The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 6.0).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse HPX on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse HPX is greater than 95% as determined by SEC-HPLC.
Background :
Hemopexin (HPX) serves as scavenger and transporter of toxic plasma heme to the liver. HPX is formed by two four-bladed beta-propeller domains, resembling two thick disks that lock together at a 90 degrees angle. The heme is bound between the two beta-propeller domains in a pocket formed by the interdomain linker peptide.HPX, acting not only as a heme carrier but also displaying transient heme-based ligand binding and (pseudo-)enzymatic properties, could be considered a ‘chronosteric’ heme-protein.
Synonyms :
Hemopexin;Hpx;Hpxn
References & Citations :
(1) Ascenzi P, Fasano M. Heme-hemopexin: a ‘chronosteric’ heme-protein. IUBMB Life. 2007 Nov;59(11):700-8. doi: 10.1080/15216540701689666. PMID: 17968708.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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