Name : Human HRG/HPRG Protein
Product Source :
Recombinant Human HRG/HPRG Protein is expressed from HEK293 with His tag at the C-terminus. It contains Val19-Lys525.[Accession | NP_000403.1]
Molecular Weight :
The protein has a predicted MW of 58.75 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human HRG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human HRG is greater than 95% as determined by SEC-HPLC.
Background :
Histidine-rich glycoprotein (HRG) is a 75 kDa glycoprotein synthesized in the liver whose plasma concentration is 100-150 μg/ml. HRG has been shown to modulate sepsis-related biological reactions by binding to several substances and cells, including heparin, factor XII, fibrinogen, thrombospondin, plasminogen, C1q, IgG, heme, LPS, dead cells, bacteria, and fungi.
Synonyms :
HRG; HPRG; THPH11
References & Citations :
Wake H. Histidine-rich Glycoprotein Modulates the Blood-vascular System in Septic Condition. Acta Med Okayama. 2019 Oct;73(5):379-382. doi: 10.18926/AMO/57366. PMID: 31649362.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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