Name : Human HTRA1 Protein

Product Source :
Recombinant Human HTRA1 Protein is expressed from E.coli with His tag at the C-Terminus. It contains Gln23-Pro480.[Accession | Q92743]

Molecular Weight :
The protein has a predicted MW of 50.14 kDa. The protein migrates to 38-40 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Supplied as 0.22μm filtered solution in 50mM Tris, 500mM NaCl (pH 8.5).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human HTRA1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. Bioactivity Data Measured by its ability to cleave beta-casein.R1: Incubate Human HTRA1 and beta-casein at 37 °C for 20 minutes.R2: Incubate beta-casein at 37 °C for 20 minutes.R3: Keep the beta-casein frozen at -20 °C for 20 minutes.

Background :
HtrA1 (High temperature requirement A1) family proteins include four members, widely conserved from prokaryotes to eukaryotes, named HtrA1, HtrA2, HtrA3 and HtrA4. HtrA1 is a serine protease involved in a variety of biological functions regulating many signaling pathways degrading specific components and playing key roles in many human diseases such as neurodegenerative disorders, pregnancy complications and cancer.

Synonyms :
L56; Serine protease 11; HTRA; PRSS11; 5654; ARMD7; IGFBP5-protease; L56; ORF480;

References & Citations :
Tossetta G, Fantone S, Licini C, Marzioni D, Mattioli-Belmonte M. The multifaced role of HtrA1 in the development of joint and skeletal disorders. Bone. 2022 Apr;157:116350. doi: 10.1016/j.bone.2022.116350. Epub 2022 Feb 5. PMID: 35131488.

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