Name : Human ICAM-1/CD54 Protein
Product Source :
Recombinant Human ICAM-1/CD54 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln28-Glu480.[Accession | AAH15969]
Molecular Weight :
The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ICAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ICAM-1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human ICAM-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.
Background :
Intercellular adhesion molecule-1 (ICAM-1; CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis.
Synonyms :
ICAM-1; BB2; CD54
References & Citations :
(1) Lawson C, Wolf S. ICAM-1 signaling in endothelial cells. Pharmacol Rep. 2009 Jan-Feb;61(1):22-32. doi: 10.1016/s1734-1140(09)70004-0. PMID: 19307690.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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