Name : Human IFN gamma/IFNG Protein

Product Source :
Recombinant Human IFN gamma/IFNG Protein is expressed from E.coli without tag. It contains Gln24-Gln166.[Accession | P01579]

Molecular Weight :
The protein has a predicted MW of 16.77 kDa same as Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in 50mM Tris (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human IFN gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IFN gamma is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IFN gamma, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 27.6ng/ml determined by ELISA.

Background :
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.

Synonyms :
Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma; IFNγ

References & Citations :
(1)Ikeda H, Old LJ, Schreiber RD. The roles of IFN gamma in protection against tumor development and cancer immunoediting. Cytokine Growth Factor Rev. 2002;13(2):95-109. doi:10.1016/s1359-6101(01)00038-7.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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