Name : Human IGFBP2 Protein

Product Source :
Recombinant Human IGFBP2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala36-Gln325.[Accession | P18065]

Molecular Weight :
The protein has a predicted MW of 32.5 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human IGFBP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IGFBP2 is greater than 95% as determined by SEC-HPLC.

Background :
Insulin-like growth factor (IGF) binding protein 2 (IGFBP2) was discovered and identified as an IGF system regulator, controlling the distribution, function, and activity of IGFs in the pericellular space. IGFBP2 is a developmentally regulated gene that is highly expressed in embryonic and fetal tissues and markedly decreases after birth. Aberrant expression of IGFBP2 in cancer acts as a hub of an oncogenic network, integrating multiple cancer signaling pathways and serving as a potential therapeutic target for cancer treatment.

Synonyms :
IBP-2; IGFBP-2; BP2; AI255832; mIGFBP-2; IGFBP2; IGF-BP53

References & Citations :
(1) Li T, Forbes ME, Fuller GN, Li J, Yang X, Zhang W. IGFBP2: integrative hub of developmental and oncogenic signaling network. Oncogene. 2020 Mar;39(11):2243-2257. doi: 10.1038/s41388-020-1154-2. Epub 2020 Jan 10. PMID: 31925333; PMCID: PMC7347283.

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