Name : Human IGFII Protein
Product Source :
Recombinant Human IGFII Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ala25-Glu91.[Accession | P01344-1]
Molecular Weight :
The protein has a predicted MW of 33.00 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IGFII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IGFII is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IGFBP-3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA.
Background :
The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins. All three ligands are involved in cell proliferation, apoptosis, protein synthesis and metabolism due to their homologous sequences and structural similarities. Insulin-like growth factor 2, a member of the insulin family, plays an important role in embryonic development, metabolic disorders, and tumorigenesis by combining with three receptors with different degrees of affinity.
Synonyms :
IGF-II; Somatomedin-A; Somatomedin A; IGF2; PP1446
References & Citations :
Zhu Y, Chen L, Song B, Cui Z, Chen G, Yu Z, Song B. Insulin-like Growth Factor-2 (IGF-2) in Fibrosis. Biomolecules. 2022 Oct 25;12(11):1557. doi: 10.3390/biom12111557. PMID: 36358907; PMCID: PMC9687531.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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