Name : Human IgG1 Fc Protein
Product Source :
Recombinant Human IgG1 Fc is expressed from HEK293 without tag. It contains Asp104-Lys330.[Accession | P01857-1]
Molecular Weight :
The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IgG1 Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IgG1 Fc is greater than 95% as determined by SEC-HPLC. SPR Data Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 μM as determined in SPR assay (Biacore T200).
Background :
IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Synonyms :
IgG1; Igh-4; RGD1359539; VH7183
References & Citations :
(1)Ponstingl H, Hilschmann N. [Rule of antibody structure. The primary structure of a monoclonal IgG1 immunoglobulin (myeloma protein Nie), II: isolation and amino acid sequence of the tyrptic peptides from the H-chain (author’s transl)].[J]. Hoppe Seylers Zeitschrift Für Physiologische Chemie, 1976, 357(11):1541.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
NXPH1 Protein
EDAR Protein
Popular categories:
Receptor Guanylyl Cyclase Family
B7-DC/CD273