Name : Mouse IgG2a Fc Protein
Product Source :
Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag. It contains Glu98-Lys330.[Accession | P01863]
Molecular Weight :
The protein has a predicted MW of 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse IgG2a Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse IgG2a Fc is greater than 95% as determined by SEC-HPLC. SPR Data Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).
Background :
Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life.
Synonyms :
IgG2a; IgG2a-Fc; Igh-1; Igh 1; Immunoglobulin G2A Fc; G2A Fc
References & Citations :
[1] Jacobson BA, Rothstein TL, Marshak-Rothstein A. Unique site of IgG2a and rheumatoid factor production in MRL/lpr mice. Immunol Rev. 1997 Apr;156:103-10.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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