Name : Rabbit IgG Protein
Product Source :
Recombinant Rabbit IgG Protein is expressed from HEK293 without tag. It contains Ser101-Lys323 (T185A, N284S).[Accession | P01870]
Molecular Weight :
The protein has a predicted MW of 25.13 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS, 150mM Nacl, 10% Glycerol (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Rabbit IgG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Rabbit IgG is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Rabbit IgG, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5ng/ml determined by ELISA.
Background :
Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus.
Synonyms :
IgG;
References & Citations :
(1) Kao D, Lux A, Schaffert A, Lang R, Altmann F, Nimmerjahn F. IgG subclass and vaccination stimulus determine changes in antigen specific antibody glycosylation in mice. Eur J Immunol. 2017 Dec;47(12):2070-2079. doi: 10.1002/eji.201747208. Epub 2017 Aug 29. PMID: 28771702.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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