Name : Mouse AARS1 Protein

Product Source :
Recombinant Mouse AARS1 Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus. It contains Met1-Asn968.[Accession | Q8BGQ7]

Molecular Weight :
The protein has a predicted MW of 108.43 kDa same as Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse AARS1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Background :
Alanyl-tRNA synthetase 1 (AARS1) gene encodes a ubiquitously expressed class II enzyme that catalyzes the attachment of alanine to the cognate tRNA. AARS1 mutations are frequently responsible for autosomal dominant Charcot-Marie-Tooth disease type 2N (CMT2N).

Synonyms :
AlaRS; Sti; Aars1; Aars

References & Citations :
Lee AJ, Nam DE, Choi YJ, Nam SH, Choi BO, Chung KW. Alanyl-tRNA synthetase 1 (AARS1) gene mutation in a family with intermediate Charcot-Marie-Tooth neuropathy. Genes Genomics. 2020 Jun;42(6):663-672. doi: 10.1007/s13258-020-00933-9. Epub 2020 Apr 20. PMID: 32314272.

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