Product Source :
Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed from HEK293 without tag. It contains Phe14-Glu343.[Accession | NP_004624.1]
Molecular Weight :
The protein has a predicted MW of 37.78 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-1R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-1R2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-1R2, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.21μg/ml determined by ELISA.
Background :
Interleukin (IL)-1 has been reported to be involved in the development of tuberculosis (TB). IL1R1 and IL1R2 encode a cytokine receptor that belongs to the IL-1 receptor family.IL1R2 (Interleukin 1 Receptor Type 2) is a Protein Coding gene. Diseases associated with IL1R2 include Endometriosis and Mastitis. Among its related pathways are Hematopoietic cell lineage and IL-1 signaling pathway. Gene Ontology (GO) annotations related to this gene include interleukin-1 receptor activity and interleukin-1, type II, blocking receptor activity.
Synonyms :
IL-1R-2; IL-1RT-2; IL-1RT2; CDw121b; IL-1R-beta; CD121b; IL1RB; IL1R2; IL-1 RII; MGC47725; IL-1R-β
References & Citations :
(1)Zhang L, Qiang J, Yang X, et al. IL1R2 Blockade Suppresses Breast Tumorigenesis and Progression by Impairing USP15-Dependent BMI1 Stability. Adv Sci (Weinh). 2019;7(1):1901728. Published 2019 Nov 13. doi:10.1002/advs.201901728
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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