Name : Human IL-13 Protein
Product Source :
Recombinant Human IL-13 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Gly21-Asn132.[Accession | AAK53823]
Molecular Weight :
The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-13 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human IL-13Ra1, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test). SPR Data Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 μM as determined in SPR assay (Biacore T200).
Background :
Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
Synonyms :
IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
References & Citations :
(1)Grünig G, Corry D B , Reibman J , et al. Interleukin 13 and the evolution of asthma therapy[J]. American Journal of Clinical & Experimental Immunology, 2012, 1(1):20.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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