Name : Human IL-10 R alpha Protein
Product Source :
Recombinant Human IL-10 R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains His22-Asn235.[Accession | Q13651]
Molecular Weight :
The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-10 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-10 R alpha is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 48.4ng/ml determined by ELISA (QC Test). SPR Data Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Background :
The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne’s disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.
Synonyms :
IL-10RA; IL10RA; IL10R; IL-10R1; IL-10R subunit 1; CDw210a; HIL-10R; IBD28; IL-10 R alpha
References & Citations :
(1) Mallikarjunappa S, Shandilya UK, Sharma A, Lamers K, Bissonnette N, Karrow NA, Meade KG. Functional analysis of bovine interleukin-10 receptor alpha in response to Mycobacterium avium subsp. paratuberculosis lysate using CRISPR/Cas9. BMC Genet. 2020 Nov 2;21(1):121. doi: 10.1186/s12863-020-00925-4. PMID: 33138773; PMCID: PMC7607837.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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