Name : Human IL-1R3/IL-1 RAcP Protein
Product Source :
Recombinant Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser21-Glu359.[Accession | Q9NPH3-1]
Molecular Weight :
The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-1R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-1R3 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-1R3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
Background :
IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.
Synonyms :
IL1RAP; IL-1RAP; C3orf13; IL-1 R3; IL-1 RAcP
References & Citations :
(1)gerstam, Helena, Karlsson C , Hansen N , et al. Antibodies targeting human IL1RAP (IL1R3) show therapeutic effects in xenograft models of acute myeloid leukemia[J]. Proceedings of the National Academy of Sciences, 2015, 112(34):10786-10791.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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