Name : Mouse IL-12 Protein
Product Source :
Recombinant Mouse IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg23-Ala215(IL-12A)&Met23-Ser335(IL-12B).[Accession | P43431(IL-12A)&P43432(IL-12B)]
Molecular Weight :
The protein has a predicted MW of 22.80 kDa(IL-12A)&35.79 kDa(IL-12B). Due to glycosylation, the protein migrates to 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse IL-12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse IL-12 is greater than 95% as determined by SEC-HPLC. Cell Based Assay Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.5 – 2 ng/mL.
Background :
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Synonyms :
CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12
References & Citations :
(1) Lu X. Impact of IL-12 in Cancer. Curr Cancer Drug Targets. 2017;17(8):682-697. doi:10.2174/1568009617666170427102729
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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