Name : Human IL-22 Protein
Product Source :
Recombinant Human IL-22 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Ala34-Ile179.[Accession | Q9GZX6]
Molecular Weight :
The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-22 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-22, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human IL-22, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA.
Background :
The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
Synonyms :
Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18
References & Citations :
(1) Keir M, Yi Y, Lu T, Ghilardi N. The role of IL-22 in intestinal health and disease. J Exp Med. 2020 Feb 13;217(3):e20192195. doi: 10.1084/jem.20192195. PMID: 32997932; PMCID: PMC7062536.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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