Name : Cynomolgus IL-4 Protein
Product Source :
Recombinant Cynomolgus IL-4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains His25-Ser153.[Accession | P79339-1]
Molecular Weight :
The protein has a predicted MW of 16.03 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus IL-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus IL-4 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus IL-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). SPR Data Cynomolgus IL-4, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).
Background :
Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. .
Synonyms :
IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1
References & Citations :
(1)Ghoreschi K , Thomas P , Breit S , et al. Interleukin-4 therapy of psoriasis induces Th2 responses and improves human autoimmune disease[J]. Nature Medicine, 2002, 9(1):40-46.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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