Name : Cynomolgus IL-5 Protein
Product Source :
Recombinant Cynomolgus IL-5 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Ile20-Ser134.[Accession | A0A2K5U1E7]
Molecular Weight :
The protein has a predicted MW of 14.25 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus IL-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus IL-5 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus IL-5, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA.
Background :
IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
Synonyms :
Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
References & Citations :
(1) Hassani M, Koenderman L. Immunological and hematological effects of IL-5(Rα)-targeted therapy: An overview. Allergy. 2018 Oct;73(10):1979-1988. doi: 10.1111/all.13451. Epub 2018 Oct 8. PMID: 29611207; PMCID: PMC6220846.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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