Name : Human IL-15 Protein
Product Source :
Recombinant Human IL-15 Protein is expressed from E.coli without tag. It contains Asn49-Ser162.[Accession | P40933]
Molecular Weight :
The protein has a predicted MW of 12.8 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by SEC-HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-15 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 22.1ng/ml determined by ELISA. SPR Data Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9nM as determined in SPR assay (Biacore T200). Cell Based Assay Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 1-2 ng/mL. The specific activity of recombinant human IL-15 is > 5 x 10^7 units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test). Batch to Batch Consistency Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Bioactivity of three different batches of Human IL-15 shows very high batch-to-batch consistency.
Background :
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases.IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
References & Citations :
(1)Rautela J , Huntington N D. IL-15 signaling in NK cell cancer immunotherapy[J]. Current Opinion in Immunology, 2017, 44:1-6.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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