Name : Human IL-37 Protein
Product Source :
Recombinant Human IL-37 Protein is expressed from E.coli without tag. It contains Lys27-Asp192.[Accession | Q9NZH6-2]
Molecular Weight :
The protein has a predicted MW of 18.55 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM PBS, 2mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-37 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-37 is greater than 95% as determined by SEC-HPLC. SPR Data Human IL-18BP, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-37, No Tag with an affinity constant of 0.12 μM as determined in SPR assay (Biacore T200).
Background :
IL-37 is a unique member of the IL-1 family of cytokines, which functions as a natural suppressor of inflammatory and immune responses. Immune and non-immune cells produce IL-37 precursor following pro-inflammatory stimuli. Following activating cleavage by caspase-1, mature IL-37 translocates to the nucleus, where it suppresses transcription of pro-inflammatory genes.
Synonyms :
FIL1 zeta; IL-1X; IL-1F7; IL-1H; IL-1H4; IL-1 zeta; IL-1RP1; FIL1Z; IL1F7; IL1H4; IL1RP1
References & Citations :
Cavalli G, Dinarello CA. Suppression of inflammation and acquired immunity by IL-37. Immunol Rev. 2018 Jan;281(1):179-190. doi: 10.1111/imr.12605. PMID: 29247987.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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